PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli

Authors
Solaiman, DKY
Citation
Dky. Solaiman, PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli, BIOTECH LET, 22(9), 2000, pp. 789-794
Citations number
20
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
9
Year of publication
2000
Pages
789 - 794
Database
ISI
SICI code
0141-5492(200005)22:9<789:PCOPRP>2.0.ZU;2-4
Abstract
A ca. 5.5-kb region of Pseudomonas resinovorans genome containing the polyh ydroxyalkanoate (PHA) biosynthesis locus was sequenced. Three complete open -reading-frames (ORFs), i.e., phaCl(Pr), phaZ(Pr), and phaC2(Pr), were iden tified. Using this sequence information, phaCl(Pr) was PCR-cloned from P. r esinovorans genomic DNA and expressed in E. coli as shown by a Nile Red pla te assay and gas chromatography/mass spectrometric analysis.