Hj. Yoon et al., Induced accumulation of triterpenoids in Scutellaria baicalensis suspension cultures using a yeast elicitor, BIOTECH LET, 22(13), 2000, pp. 1071-1075
When growth-phase cell suspension cultures of Scutellaria baicalensis were
treated with 50 mu g of yeast elicitor preparation ml(-1), both oleanolic a
cid and ursolic acid transiently increased in the culture medium rather tha
n in the cells. The maximal triterpenoid concentration was 13.7 mg l(-1) me
dia approx. 35 h after treatment, whereas the maximum concentration was 2.1
mg l(-1) media after about 20 h following treatment with methyl jasmonate.
Elicitor treatment also doubled phospholipase A(2) activity (25 pmol mg(-1
) min) and the simultaneous treatment of aristolochic acid, a phospholipase
A(2) inhibitor, inhibited triterpenoids accumulation as well as phospholip
ase A(2) activity.