A simple method to determine urea concentration using intact Helicobacter pylori and BromoCresol Purple as a pH indicator

Authors
Lin, YL Chen, CT Lin, SC Lee, C Kuo, HS Shih, CM Hsu, YH Chin, YP Chan, EC
Citation
Yl. Lin et al., A simple method to determine urea concentration using intact Helicobacter pylori and BromoCresol Purple as a pH indicator, BIOTECH LET, 22(13), 2000, pp. 1077-1079
Citations number
9
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
13
Year of publication
2000
Pages
1077 - 1079
Database
ISI
SICI code
0141-5492(200007)22:13<1077:ASMTDU>2.0.ZU;2-Y
Abstract
A modified method for urea quantification, by measuring the ammonia formed by urease, used the urease-positive Helicobacter pylori in place of purifie d urease with a pH indicator dye, BromoCresol Purple, to provide a color ch ange. The color formed was stable for 20 min and could be read at 588 nm fo r urea quantification. Using this method, urea standard curves were linear up to 8.3 mM. As there was no need for centrifugation or precipitation, the assay was developed for use with 96-well microplates.