Citation
Sc. Ribeiro et al., Quantitation of plasmid DNA in aqueous two-phase systems by fluorescence analysis, BIOTECH LET, 22(13), 2000, pp. 1101-1104
Citations number
8
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492
→ ACNP
Volume
22
Issue
13
Year of publication
2000
Pages
1101 - 1104
Database
ISI
SICI code
0141-5492(200007)22:13<1101:QOPDIA>2.0.ZU;2-8
Abstract
The development of aqueous two-phase systems for plasmid purification from
Escherichia coli cell lysates requires a reliable DNA quantitation method.
Plasmid DNA was quantified by fluorescence using PicoGreen nucleic acid sta
in. Linearity was obtained up to 40 ng plasmid ml(-1). Two polyethyleneglyc
ol (PEG)/salt systems were studied, PEG 600/K2HPO4 and PEG 300/K2HPO4. The
average plasmid recovery was 41% in the bottom phase of the first system an
d 35% in the top phase of the second system. This method has proved to be s
imple and reproducible.