Enzymatic preparation of a carbohydrate ester of medium-chain fatty acid in supercritical carbon dioxide

Authors
Heo, JH Kim, SY Kim, HS Yoo, KP
Citation
Jh. Heo et al., Enzymatic preparation of a carbohydrate ester of medium-chain fatty acid in supercritical carbon dioxide, BIOTECH LET, 22(12), 2000, pp. 995-998
Citations number
10
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
12
Year of publication
2000
Pages
995 - 998
Database
ISI
SICI code
0141-5492(200006)22:12<995:EPOACE>2.0.ZU;2-F
Abstract
Acylation of methyl-beta-d-fructofuranoside and caprylic acid with an immob ilized lipase, Novozym 435, was carried out in supercritical carbon dioxide (SCCO2) with tert-butanol as a co-solvent. Initial rate of acylation was 1 2-fold higher in SCCO2 than in tert-butanol. The equilibrium conversion was increased up to 70% with an increase in the molar ratio up to a maximum of 1:20 (methyl-beta-d-fructofuranoside:caprylic acid) at 16 MPa and 70 degre es C.