Gene delivery for genetically engineered mucosal cells with enhanced function

Authors
Nagatani, N Shinkai, M Nagase, Y Honda, H Hata, K Mizuno, H Ueda, M Kobayashi, T
Citation
N. Nagatani et al., Gene delivery for genetically engineered mucosal cells with enhanced function, BIOTECH LET, 22(12), 2000, pp. 999-1002
Citations number
7
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
12
Year of publication
2000
Pages
999 - 1002
Database
ISI
SICI code
0141-5492(200006)22:12<999:GDFGEM>2.0.ZU;2-A
Abstract
A non-viral transfection method for oral mucosal cells was investigated usi ng a modified transfection method and five commercial transfection reagents . The CellFECTIN(TM) gave the highest expression of a transfected gene. Whe n the mucosal cells were transfected with 0.3 ng DNA/cell, the transfection efficiency was optimal, and the production of a reporter protein increased up to ten times higher than those with the other transfection reagents.