One-step partial purification of Solanum tuberosum tuber lectin using magnetic chitosan particles

Authors
Safarikova, M Safarik, I
Citation
M. Safarikova et I. Safarik, One-step partial purification of Solanum tuberosum tuber lectin using magnetic chitosan particles, BIOTECH LET, 22(11), 2000, pp. 941-945
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
11
Year of publication
2000
Pages
941 - 945
Database
ISI
SICI code
0141-5492(200006)22:11<941:OPPOST>2.0.ZU;2-W
Abstract
A one-step procedure for a partial purification of Solanum tuberosum tuber lectin has been developed. Lectin from tuber extract or from potato wastewa ter was adsorbed to magnetic chitosan particles and eluted with low pH buff er. The specific activity of separated lectin increased 13 times during the purification process and the recovery was 50%.