Alkyl-beta-glucosides were synthesized with beta-glucosidase, from almond,
in an amphiphilic phase reaction system using different water-miscible coso
lvents. By means of stepwise addition of glucose, we obtained about 1.5 tim
es higher concentration of hexylglucoside than that of normal reaction. Usi
ng the same way, octyl-, decyl- and dodecylglucoside were synthesized and t
heir concentrations were 6.64, 5.3 and 4.48 g l(-1), respectively.