A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin

Authors
Chen, ZL Iding, K Lutkemeyer, D Lehmann, J
Citation
Zl. Chen et al., A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin, BIOTECH LET, 22(10), 2000, pp. 837-841
Citations number
10
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
10
Year of publication
2000
Pages
837 - 841
Database
ISI
SICI code
0141-5492(200005)22:10<837:ALCDPF>2.0.ZU;2-1
Abstract
A chemically defined protein free medium, DF6S, was developed for the culti vation of a recombinant Chinese hamster ovary cell line (CHO2DS) producing human prothrombin in suspension batch culture. DF6S was formulated by optim izing DME/F12 with amino acids and supplementing the optimized DME/F12 with aurintricarboxylic acid, ethanolamine, ferric sulfate, Pluronic F68, putre scine and sodium pyruvate. From a seeding density of 2.3 x 10(5) cells ml(- 1), CHO2DS cells grown in suspension in DF6S medium reached a maximal cell density of 1.92 x 10(6) cells ml(-1) with an accumulated prothrombin concen tration of 16.7 mg l(-1) after 6 days in culture.