Transport and consumption rate of O-2 in alginate gel beads entrapping hepatocytes

Authors
Annesini, MC Castelli, G Conti, F De Virgiliis, LC Marrelli, L Miccheli, A Satori, E
Citation
Mc. Annesini et al., Transport and consumption rate of O-2 in alginate gel beads entrapping hepatocytes, BIOTECH LET, 22(10), 2000, pp. 865-870
Citations number
19
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
10
Year of publication
2000
Pages
865 - 870
Database
ISI
SICI code
0141-5492(200005)22:10<865:TACROO>2.0.ZU;2-E
Abstract
Experiments carried out with hepatocytes entrapped in alginate gel particle s with a cell density of about 10(7) cells ml(-1) showed an oxygen consumpt ion rate of about 2.2x10(-16) mol cell(-1) s(-1). Under these conditions, n o inner anoxic core is present in 1.8 mm diameter beads. From these data, t he maximum bead size consistent with non-critical O-2 concentration at the bead center has been evaluated for different cell densities and O-2 concent rations in the medium.