Preparation of vinyl thymidine ester catalyzed by protease and its chemical polymerization

Authors
Kitagawa, M Fan, H Raku, T Kurane, R Tokiwa, Y
Citation
M. Kitagawa et al., Preparation of vinyl thymidine ester catalyzed by protease and its chemical polymerization, BIOTECH LET, 22(10), 2000, pp. 883-886
Citations number
16
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
10
Year of publication
2000
Pages
883 - 886
Database
ISI
SICI code
0141-5492(200005)22:10<883:POVTEC>2.0.ZU;2-U
Abstract
Transesterification reaction of 0.25 M thymidine with 1 M divinyladipate in dimethylformamide (DMF) was catalyzed by an alkaline protease (5 mg ml(-1) ) from Streptomyces sp. (20 units mg(-1) min) at 30 degrees C for 7 days to give 5'-O-vinyladipoyl thymidine (yield 77%) without formation of any by-p roducts. Poly(vinyl alcohol) containing thymidine branches could be obtaine d by its free-radical polymerization.