Protection of hematopoietic cells against combined O-6-benzylguanine and chloroethylnitrosourea treatment by mutant forms of O-6-methylguanine DNA methyltransferase

Authors
Williams, DA Maze, R Kurpad, C Pegg, A Erickson, LC
Citation
Da. Williams et al., Protection of hematopoietic cells against combined O-6-benzylguanine and chloroethylnitrosourea treatment by mutant forms of O-6-methylguanine DNA methyltransferase, BONE MAR TR, 25, 2000, pp. S105-S109
Citations number
35
Categorie Soggetti
Hematology,"Medical Research Diagnosis & Treatment
Journal title
BONE MARROW TRANSPLANTATION
ISSN journal
02683369 → ACNP
Volume
25
Year of publication
2000
Supplement
2
Pages
S105 - S109
Database
ISI
SICI code
0268-3369(200005)25:<S105:POHCAC>2.0.ZU;2-7
Abstract
This report demonstrates that expression of the P140A O-6-methylguanine DNA methyl transferase (MGMT) mutant via retrovirus-mediated gene transfer lea ds to significant, but modest, resistance of cells to both 6-benzylguanine (6-BG) depletion and treatment with 1,3-bis (2-chloroethyl)-1-nitrosourea ( BCNU), Expression of the P140A/G156A double mutant appeared to be associate d with reduced or unstable protein in hematopoietic cells.