The inhibition of cholera toxin-induced 5-HT release by the 5-HT3 receptorantagonist, granisetron, in the rat

1 The secretagogue 5-hydroxytryptamine (5-MT) is implicated in the pathophy siology of cholera. 5-HT released from enterochromaffin cells after cholera toxin exposure is thought to activate non-neuronally (5-HT2 dependent) and neuronally (5-HT3 dependent) mediated water and electrolyte secretion. CT- secretion can be reduced by preventing the release of 5-MT. 2 Enterochromaffin cells possess numerous receptors that, under basal condi tions, modulate 5-HT release. These include basolateral 5-HT3 receptors, th e activation of which is known to enhance 5-HT release. 3 Until now, 5-HT3 receptor antagonists (e.g. granisetron) have been though t to inhibit cholera toxin-induced fluid secretion by blockading 5-HT3 rece ptors on secretory enteric neurones. Instead we postulated that they act by inhibiting cholera toxin-induced enterochromaffin cell degranulation. 4 Isolated intestinal segments in anaesthetized male Wistar rats, pre-treat ed with granisetron 75 mu g kg(-1), lidoocaine 6 mg kg(-1) or saline, were instilled with a supramaximal dose of cholera toxin or saline. Net fluid mo vement was determined by small intestinal perfusion or gravimetry and small intestinal and luminal fluid 5-HT levels were determined by HPLC with fluo rimetric detection. 5 Intraluminal 5-HT release was proportional to the reduction in tissue 5-H T levels and to the onset of water and electrolyte secretion, suggesting th at luminal 5-HT levels reflect enterochromaffin cell activity. 6 Both lidocaine and granisetron inhibited fluid secretion. However, granis etron alone, and proportionately, reduced 5-HT release. 7 The simultaneous inhibition of 5-HT release and fluid secretion by granis etron suggests that 5-HT release from enterochromaffin cells is potentiated by endogenous 5-MT, accentuated 5-HT release promotes cholera toxin-induce d fluid secretion.