Separation of polynuclear aromatic hydrocarbons and phenols on a new chemically bonded phase for high-performance liquid chromatograghy

A new bonded liquid-crystal stationary phase for highperformance liquid chr omatography is presented. Synthesis entailed reaction of gamma-benzyl-L-glu tamate-N-carboxyanhydride with aminopropyl LiChrospher 100 NH2. The new sta tionary phase was characterized by solid-state C-13 NMR and elemental analy sis. Its analytical properties were tested with polynuclear aromatic hydroc arbons (PAH) and phenols. PAH were chromatographed with hexane-chloroform m ixtures as mobile phases and were eluted in order of increasing ring number . Total resolution of the 22 PAH studied was achieved by gradient elution. Phenols were chromatographed with a mixture of chloroform and methanol as m obile phase. Small amounts of methanol have a substantial effect on the sep aration of the phenols. Use of 0.5% (nu/nu) methanol enabled the separation of 19 phenols and phenol derivatives.