Aloe L. - a second plant family without (TTTAGGG)(n) telomeres

The physical ends of chromosomes are protected and stabilised by telomeres. The sequence of telomeric DNA normally consists of a simple repeating unit that is conserved in many organisms. Most plants examined have been shown to possess Arabidopsis-type telomeres consisting of many repeat copies of t he sequence 5'-TTTAGGG-3'. Using fluorescent in situ hybridisation, slot bl otting and the asymmetric polymerase chain reaction we demonstrate an absen ce of Arabidopsis-type telomeres in the genus Aloe (family Asphodelaceae). The only other plant genera so far reported without such telomeres are Alli um, Nothoscordum, and Tulbaghia (family Alliaceae). As these genera and Alo e are petal-oid monocots in the Asparagales, it is suggested that an absenc e of Arabidopsis-type telomeres may be characteristic of this related group of plants.