The structure of the carbohydrate backbone of the lipopolysaccharides fromBordetella hinzii and Bordetella bronchiseptica

The structure of the core-lipid A region of the lipopolysaccharides from Bo rdetella hinzii and Bordetella bronchiseptica has been analyzed. Lipopolysa ccharides were deacylated using strong alkaline hydrolysis, the products we re separated by high performance anion-exchange chromatography and analyzed by NMR and mass spectrometry. The following structure of the products can be deduced from the experimental results: [GRAPHICS] where for the product from Bordetella hinzii N = H, R = H, beta-FucN4N- or partially N-acetylated Sug-(1-3)-beta-FucN4N and for the product from Borde tella bronchiseptica N = alpha-Hep, R = H, beta-FucN4N, beta-FucN4NMe or pa rtially N-acetylated Sug-(1-3)-beta-FucN4N or Sug-(1-3)-beta-FucN4NMe; Sug = 2,3-diamino-2,3,4-trideoxy-hex-4-enuronopyranosyl.