H. Kato et al., Distribution of apolipoproteins A-I and A-II in serum, secreted proteins by cultured hepatocytes, and the plasma membrane of hepatocytes in eel, FISHERIES S, 66(3), 2000, pp. 579-585
Apolipoprotein A-I (apoA-I) of high-density lipoprotein (HDL) plays a key r
ole in cholesterol transport as a ligand of HDL binding proteins or HDL rec
eptors and an acceptor of intracellular cholesterol. It has been known that
the latter role of apoA-I is carried out by free apoA-I dissociated from H
DL. We investigated the distribution of free apoA-I and apoA-II in eel seru
m and secreted proteins from cultured eel hepatocytes using monoclonal anti
bodies specific to apoA-I or apoA-II of eel serum HDL. Free apoA-I was foun
d in eel serum and the secreted proteins, but free apoA-II was not. ApoA-I
and apoA-II were also found in serum very low-density lipoprotein and chylo
micron-like lipoprotein secreted by cultured eel hepatocytes. We observed t
he existence of apoA-I and apoA-II in the plasma membrane of the hepatocyte
s by immunological method after fixation of the cells. Furthermore, when th
e isolated hepatocytes treated with the antibody to apoA-II were incubated
with HDL stained by a fluorescent lipophilic dye, the binding of the HDL to
the hepatocytes was inhibited by 30%. However, we did not observe the inhi
bition by the hepatocytes treated with the antibody to apoA-I.