Use of the 6-methylsalicylic-acid-synthase gene as a discriminating markerbetween Aspergillus terreus and Aspergillus flavipes

In nineteen pathogenic and saprophytic isolates denoted as Aspergillus terr eus the presence and restriction pattern of the gene pksM (6-methylsalicyli c acid synthase) was determined. Five patterns (A-E) were found and in thre e isolates the gene was missing. The RAPD (random amplified polymorphic PNA ) patterns with three primers were analyzed. The strains with pksM pattern possibly derived from the most common pattern A by single mutation (pattern s B-D) were also related by RAPD. Among them, three clades were found. The first one contained saprophytes from Asia, the other two clades contained b oth European and American pathogens and each of them one saprophyte. The se quences of rDNA region containing 5.8S rDNA and spacers ITS1 and ITS2 were established for representatives of each group and the strains missing the p ksM gene. The isolates possessing pksM (although with different restriction patterns) grouped as A. terreus group, whereas the isolates lacking this g ene were close to Fennellia flavipes.