Codominant analysis of banding data from a dominant marker system by normal mixtures

Amplified fragment length polymorphisms (AFLPs) currently are among the mos t widely used marker systems. In many studies, AFLPs are analyzed on the ba sis of the presence or absence of a band on an electrophoretic gel. As a re sult, dominant homozygous individuals are not distinguished from heterozygo us individuals, resulting in a considerable loss of information. This artic le shows how codominant information can be obtained if the amount of PCR pr oducts is quantified. Due to measurement variation, genotyping on the basis of such information is not error-free. We propose use of normal mixture di stributions to determine the most likely genotype, given the data. The meth od is exemplified using AFLP data from sugar beet.