A high-density linkage map of the RN region in pigs

The porcine RN locus affects muscle glycogen content and meat quality. Mie previously mapped the RN locus to chromosome 15. This study describes the i dentification of polymorphisms for four class I and four class II markers l ocated in the RN region. Resource families were genotyped with F-SSCP marke rs (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGF BP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906.. The gene order is identical to the previously reported porcine RH map of the same region. The described ma p will facilitate positional cloning of the RN gene.