Polymorphisms in the HLA-linked olfactory receptor genes in the hutterites

Genes in the MHC have been associated with mate choice and odor preferences in a variety of animals. Although the role of HLA genes in human mate choi ce has been controversial, studies in the Hutterites have demonstrated fewe r than expected numbers of couples who match for an HLA haplotype, suggesti ng that in this population there is avoidance of mates with HLA haplotypes similar to one's own haplotype. Recently, 18 olfactory receptor (OR) genes have been mapped to the HLA region, telomeric to the HLA-F locus, providing a potential mechanism for HLA-based odor recognition and perhaps mate pref erences in humans. We screened a sample of Hutterites with diverse HLA hapl otypes for polymorphisms in the HLA-linked olfactory receptor gene, FAT11, by sequencing, denaturing high performance liquid chromatography, and allel e-specific oligo dot-blotting. Three single nucleotide polymorphisms were d etected in the single translated exon of this gene, all of which resulted i n amino acid substitutions (Phe587Leu, Ala642Val, and Thr1157Ala). The FAT1 1 Phe587-Val642-Ala1157 allele occurred on 17 different HLA haplotypes, the Leu587-Ala642-Ala1157 allele on 15 haplotypes, the Phe587-Ala642-Ala1157 a llele on 16 haplotypes, and the Phe587-Ala642-Thr1157 allele on a single ha plotype. Thus, four alleles of the FAT11 gene are present in the Hutterites . This level of variation in the FAT11 gene alone may not be sufficient to contribute to the observed patterns of mate choice in the Hutterites and to individual variation in odor preferences. Human Immunology 61, 711-717 (20 00). (C) American Society for Histocompatibility and Immunogenetics, 2000. Published by Elsevier Science Inc.