We propose a multi-level concept for fish-based assessment (MuLFA) of the e
cological integrity of running waters. This concept is designed for large-s
cale monitoring programmes such as required for the proposed Water Framewor
k Directive of the EU. Out of five different biological organisation levels
(fauna, community, guild, population and individual), we propose seven cri
teria: River-type-specific species, species with self-sustaining population
s, fish region, number of guilds, guild composition, population size and po
pulation age structure. The principle of the MuLFA is based on assessing th
e deviation from undisturbed reference conditions. Reference conditions hav
e to be compiled for every distinct river type using historical fish and ab
iotic data, present river-type-specific reference sites and reference model
s. The final assessment procedure is done by comparing the assessment reach
with the reference conditions using a 5-tiered normative scheme and assign
ing that reach to the level of highest coincidence. The benefit of the MuLF
A is its potential for consistent sensitivity to low- and high-dose human a
lterations, and due to its general character, its adaptability to all river
types.