Ya. Antonov et Aa. Soshinsky, Interactions and compatibility of ribuloso-1,5-bisphosphate carboxylase/oxygenase from alfalfa with pectin in aqueous medium, INT J BIO M, 27(4), 2000, pp. 279-285
Citations number
27
Categorie Soggetti
Biochemistry & Biophysics
Journal title
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
HPLC, dynamic light scattering, CD- and fluorescent spectroscopy, and phase
analysis methods are used to study the effect of Coulomb and non-Coulomb i
nteractions between alfalfa rubisco and pectin on their thermodynamic compa
tibility. In the acid region of pH, water insoluble interpolymer complexes
stabilized by both the Coulomb and non-Coulomb bonds are formed. In the neu
tral and alkaline regions, the complexes soluble in water are formed via no
n-Coulomb bonds, due to both the hydrophobic interaction involving the este
r groups of pectin and the hydrogen bonding between dissimilar molecules. T
he compatibility of these biopolymers is sensitive to the esterification de
gree of pectin. With the latter increasing, compatability increases at neut
ral pH, but decreases considerably in the acidic region. (C) 2000 Elsevier
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