Protein and solvent dynamics in hydrated Crambin

In this paper we present an high resolution Incoherent Quasi-elastic Neutro n Scattering experiment on Grambin hydrated at h = 0.6g/g. Hydration was at tained by using D2O and H2O, in order to separate the contribution of the p rotein hom that of the water. The spectra were interpreted iii terms of an elastic peal; and a quasi-elastic lorentzian contribution. From the obtaine d EISF information about the number of fixed and mobile hydrogens are deriv ed. For the latter, an estimation of the spanned spatial and temporal scale s is provided. In the case of protein dynamics, the obtained results favour ably compare with literature data on the conformational disorder found by X -ray diffraction on the crystalline sample. In the case of water dynamics, the data show a confinement effect on the diffusion of hydration water.