Separation of vitamins by supercritical fluid chromatography with water-modified carbon dioxide as the mobile phase

Supercritical fluid chromatography (SFC) has become a technique for solving problems that are difficult to be monitored by other chromatographic metho ds. However, the most widely used fluid, is no more polar than hexane. Pola r samples which are difficult to be analyzed with pure supercritical CO2 be cause of their high polarity can be separated by adding polar modifiers to supercritical CO2. In this paper various vitamins were well separated using water-modified supercritical CO2 fluid. The amount of water dissolved in s upercritical CO2 was measured using an amperometric microsensor made of a t hin film of perfluorosulfonate ionomer (PFSI). (C) 2000 Elsevier Science B. V. All rights reserved.