Sequence analysis of a beta-agarase gene (pjaA) from Pseudomonas sp isolated from marine environment

Authors
Lee, SB Park, JH Yoon, SC Kim, JM Kong, IS
Citation
Sb. Lee et al., Sequence analysis of a beta-agarase gene (pjaA) from Pseudomonas sp isolated from marine environment, J BIOSCI BI, 89(5), 2000, pp. 485-488
Citations number
18
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
89
Issue
5
Year of publication
2000
Pages
485 - 488
Database
ISI
SICI code
1389-1723(200005)89:5<485:SAOABG>2.0.ZU;2-1
Abstract
The pjaA gene of Pseudomonas sp. W7 consists of an open reading frame of 19 26 bp encoding beta-agarase, a protein of 642 amino acids and a molecular w eight of 69,540 Da. The expressed protein of plasmid pEAG3-3, In which 259 amino acid residues from C-terminus of the overexpression plasmid (pEAG3) w ere eliminated, led to the complete loss of agarolytic activity.