Y. Shiba et al., Effect of ethanol on the production of carboxypeptidase Y using the GAL10 promoter in a Saccharomyces cerevisiae gal80 mutant, J BIOSCI BI, 89(5), 2000, pp. 426-430
In the course of studying carboxypeptidase Y (CPY) production, we found tha
t the expression level of the gene, which is under the control of the GAL10
promoter, increased in a Saccharomyces cerevisiae gal80 mutant grown in a
medium containing ethanol as the sole carbon source. In the cultivation of
the gal80 mutant KS58-2D/pCY303 carrying a multicopy plasmid, which contain
s the PRC1 gene fused to the GAL10 promoter, CPY production continued after
the consumption of galactose. In this phase, the cells utilized ethanol as
the carbon source. To increase the CPY production level, we examined the e
ffect of carbon source feeding in a fed-batch culture. The production level
in the fed-batch culture using ethanol was 1.3-fold higher than that in a
batch culture and 1.6-fold higher than that in a fed-batch culture using ga
lactose. By 5'-deletion analysis of the GAL10 promoter, the region between
- 256 and - 232 was found to be important for the promoter activity in the
gal80 mutant growing in the presence of ethanol.