Comparison of two amplification technologies for detection and quantitation of human immunodeficiency virus type 1 RNA in the female genital tract

Citation
J. Bremer et al., Comparison of two amplification technologies for detection and quantitation of human immunodeficiency virus type 1 RNA in the female genital tract, J CLIN MICR, 38(7), 2000, pp. 2665-2669
Citations number
14
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
38
Issue
7
Year of publication
2000
Pages
2665 - 2669
Database
ISI
SICI code
0095-1137(200007)38:7<2665:COTATF>2.0.ZU;2-N
Abstract
Human immunodeficiency virus type 1 (HIV-1) RNA levels in female genital tr act and peripheral blood samples were compared using two commercial amplifi cation technologies: the Roche AMPLICOR HIV-1 MONITOR test and either the O rganon Teknika nucleic acid sequence-based amplification (NASBA-QT) assay o r the NucliSens assay. Estimates of HIV-1 RNA copy number were derived from internal kit standards and analyzed unadjusted and adjusted to a common se t of external standards. We found a discordance rate of approximately 18% b etween the two technologies for the detection of HIV-1 in either the genita l tract or peripheral blood samples. Detection discordance was not consiste nt among specimens or among women. There were no significant differences In adjusted or unadjusted estimates of HIV-1 RNA copy number in the genital t ract samples using the AMPLICOR HIV-1 MONITOR test and either the NASBA-QT assay or the NucliSens assay, In addition, the estimated HIV-1 RNA copy num ber in peripheral blood samples did not differ when tested with the NucliSe ns assay and the AMPLICOR HIV-1 MONITOR test using kit standards. However, there was a significant difference In estimated RNA copy number between the NASBA-QT assay and the AMPLICOR HIV-1 MONITOR test for internal kit standa rds, which, as we have previously shown, was eliminated after adjustment wi th the external standards. Our results suggest that the Roche and Organon T eknika assays are equivalent for quantifying HIV-1 RNA in female genital tr act specimens, although variation in detection does exist.