Detection of human cytomegalovirus DNA by real-time quantitative PCR

Authors
Nitsche, A Steuer, N Schmidt, CA Landt, O Ellerbrok, H Pauli, G Siegert, W
Citation
A. Nitsche et al., Detection of human cytomegalovirus DNA by real-time quantitative PCR, J CLIN MICR, 38(7), 2000, pp. 2734-2737
Citations number
16
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
38
Issue
7
Year of publication
2000
Pages
2734 - 2737
Database
ISI
SICI code
0095-1137(200007)38:7<2734:DOHCDB>2.0.ZU;2-S
Abstract
A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patie nts.