Wm. Gong et al., CRYSTAL-STRUCTURE DETERMINATION OF ALKALINE HAEMORRHAGIN AAH-III FROMSNAKE-VENOM OF AGKISTRODON ACUTUS, SCIENCE IN CHINA SERIES C-LIFE SCIENCES, 40(4), 1997, pp. 351-355
The haemorrhagin AaH III isolated from the snake venom of Agkistrodon
acutus is one of the few alkaline ones in snake venoms. Its crystals b
elong to space group P2(1)2(1)2(1) With a = 9.573 4 nm, b = 4.996 7 nm
and c = 4.728 8 nm. Its crystal structure was determined by the molec
ular replacement method according to the model of metalloproteinase Ad
amalysin II from eastern rattlesnake venom. The AaH III structure has
been refined by PROLSQ. The final R factor was 0.254 and the RMS devia
tions of bond lengths and angles were 0.001 8 nm and 1.5 degrees. The
structure comparison suggested that AaH III has a similar structure to
other snake venom zinc-metalloproteinases. They all belong to matrix
metalloproteinases super-family.