Cutting edge: DNA immunization with minigenes of carbohydrate mimotopes induce functional anti-carbohydrate antibody response

Citation
T. Kieber-emmons et al., Cutting edge: DNA immunization with minigenes of carbohydrate mimotopes induce functional anti-carbohydrate antibody response, J IMMUNOL, 165(2), 2000, pp. 623-627
Citations number
21
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGY
ISSN journal
00221767 → ACNP
Volume
165
Issue
2
Year of publication
2000
Pages
623 - 627
Database
ISI
SICI code
0022-1767(20000715)165:2<623:CEDIWM>2.0.ZU;2-P
Abstract
To date, the generation of anti-carbohydrate Th1 immune responses, which wo uld be useful for both tumor immunotherapy as well as in pathogen vaccine s trategies, has been elusive. To augment Th1 immune responses to carbohydrat e Ags, we describe results of DNA vaccination studies in mice using plasmid s encoding designed peptide mimotopes (minigenes) of the neolactoseries Ag Lewis Y (LeY), In contrast to LeY immunization, immunization with mimotope- encoded plasmids induced LeY cross-reactive IgG2a Abs, Minigene immunizatio n primed for a LeY-specific response that is rapidly activated upon encount er with nominal Ag upon subsequent boost, The resulting IgG2a response medi ated complement-dependent cytotoxicity of a LeY-expressing human tumor cell line in the presence of human complement. These studies establish that pep tide mimotopes of carbohydrate Ags encoded as DNA plasmids are novel immuno gens providing a means to manipulate carbohydrate cross-reactive Th1 respon ses.