Ng. Rainov et al., Characterization of a canine glioma cell line as related to established experimental brain tumor models, J NE EXP NE, 59(7), 2000, pp. 607-613
Citations number
29
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
A large animal tumor model for anaplastic glioma has been recently develope
d using immunotolerant allogeneic Beagle dogs and an established canine gli
oma cell line, J3T. This model offers advantages in terms of tumor morpholo
gy and similarity to human anaplastic glioma. The present study was aimed a
t evaluating the biological characteristics of the J3T canine glioma cell l
ine as related to experimental gene therapy studies. Furthermore, developme
nt and morphology of canine brain tumors in a xenogeneic immunodeficient SC
ID mouse model was investigated. It was demonstrated that cultured J3T cell
s can be efficiently infected by adenovirus (AV), herpes-simplex type I (HS
V), or retrovirus (RV) vectors, as well as by non-virus vectors such as cat
ionic liposome/DNA complexes. Thus, in terms of infectability and transfect
ability, J3T cells seem to be closer to human glioma than the 9L rodent gli
osarcoma. Cytotoxicity of selection antibiotics such as G418, puromycin, an
d hygromycin on J3T cells essentially resemble cytotoxicity seen with other
established glioma lines, for example, 9L, U87, or U343. RV-mediated HSV-T
K/GCV gene therapy demonstrated comparable LD,, for TK-expressing and contr
ol (non-expressing) J3T and 9L cells treated with Ganciclovir. Further, it
was proven that J3T cells are tumorigenic and may grow heterotopically and
orthotopically in a xenogeneic immunodeficient host, the SCID mouse, althou
gh morphology and growth pattern of these xenogeneic tumors differ from the
demonstrated invasive phenotype in the Beagle dog.