Effect of exercise training on erythrocyte insulin-like growth factor-I receptor binding in adolescent males

Exercise training leads to tissue anabolism by acting through the growth ho rmone (GH)-insulin-like growth factor-I (IGF-I) axis, but the role of tissu e IGF-I receptors in this process is not known, Erythrocyte IGF-I receptor binding is known to be affected by circulating levels of IGF-I. We hypothes ized that training would lead to alterations in erythrocyte IGF-I binding. Thirty-eight adolescent males (mean age 16+/-0.7 yr) were randomized to a c ontrol (n = 18) or endurance training intervention lasting 5 weeks, Erythro cyte IGF-I binding was measured by standard techniques. Quantity of recepto r binding sites (R), binding affinity constant (K-aff), and nonspecific bin ding (NS) were calculated. Training led to increases (p<0.05) in 1) K-aff x R - an index of overall binding capacity (control, 7.3 +/- 8.0%; trained, 60 +/- 21%); and 2) NS (control, 1.5 +/- 6.6%; trained 23.2 +/-7.5%). K-aff , R, and R/erythrocyte increased in trained subjects, but not significantly so. These results suggest a training-associated upregulation of IGF-I bind ing, Finally, the unexpected increase in NS may reflect IGF binding protein activity, rather than changes in the erythrocyte IGF-I receptors themselve s.