E. Waszkowska et al., Spectroscopic detection of photoproducts in lecithin model system after 8-methoxypsoralen plus UV-A treatment, J PHOTOCH B, 55(2-3), 2000, pp. 145-154
Citations number
29
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Photoreactions of 8-methoxypsoralen (8-MOP) in the presence of synthetic le
cithins esterified at the beta-position with linoleic/oleic and gamma-palmi
tic acid (PCd2/d1pal) have been studied. Following W-A (320-400 nm) irradia
tion, the photoproducts separated by thin-layer chromatography are analysed
by UV absorption spectroscopy, nuclear magnetic resonance and mass spectro
scopy. The new isolated products are lecithin double cyclobutane adducts, P
C-(8-MOP)(2), fatty acid-8-MOP split adducts from phosphatidylcholine and l
ecithin adducts with photo-oxidized 8-MOP. The photolysis performed in the
presence of 8-MOP is related to the previously reported lecithin cyclobutan
e adducts with psoralen. A hypothetical scheme of lecithin photolysis under
PUVA (psoralen + W-A) treatment is proposed. We suggest that photolysis of
lecithin may have a significant role in the chain of reactions triggered i
n cell membrane submitted to PUVA treatment. (C) 2000 Elsevier Science S.A.
All rights reserved.