Inheritance of the Anasazi pattern of partly colored seedcoats in common bean

Inheritance of Anasazi pattern of partly colored seedcoats in common bean ( Phaseolus vulgaris L,) was studied in a genetic stock t ana B V Anasazi BC3 5-593, whose Anasazi pattern is derived from Plant Introduction (PI) 45180 2, Line 5-593 is a determinate, Florida dry bean breeding line (with small black seeds) used as the recurrent parent in the development of many geneti c stocks. The F-2 from the crosstana B V Anasazi BC3 5-593 xtz virgarcus BC 3 5-593 segregated for two nonparental phenotypic classes and was consisten t with the hypothesis that a single recessive gene, with tentative symbol a na, produces the Anasazi pattern with t Z ana and a new partly colored patt ern Anabip with t z ana. Thus, the Anasazi factor is not an allele at the Z locus. Analysis of 57 random F-3 progenies from the cross t ana B V Anasaz i BC3 5-593 x tz virgarcus BC3 5-593 supported a genetic model where: 1) wi th tZ the Anasazi phenotype is controlled by a single recessive gene ana, i .e., genotype t Z ann, 2) the Anabip phenotype has the genotype tz ana, and 3) f Z/z ana produces a restricted Anasazi pattern. The allelism test cros s t z ana Anabip BC3 5-593 x t z l(ers) white BC3 5-593 produced complement ation in the F-2, demonstrating nonallelism of Ana (actually Bip) with the L locus. The allelism test cross t z ana Anabip BC3 5-593 xtz bip bipunctat a BC3 5-593 failed to show complementation in F-1 and F-2, demonstrating al lelism of Ana with the Bip locus. Using bulk segregant analysis, molecular markers Linked in coupling to the Ana (OM9(200), 5.4 cM) and Bip (OJ17(700) , 6.0 cM) genes were discovered. Allelism was also suggested by the result that the same linkage distance and recombination pattern were observed when the Ana marker was used to score the bipunctata population. We propose the gene symbol bip(ana) for the recessive allele at the Bip locus that produc es Anasazi pattern with genotype tZ bip(arm) and the Anabip pattern with ge notype tz bip(nna). Although bip(ana) and bip are both recessive to Bip, th eir interactions,with the Z locus are extraordinarily different. The patter n restrictive power of bip(ana) expresses partly colored pattern with f Z, whereas bip requires tz to express partly colored pattern.