The genome sequence of the plant pathogen Xylella fastidiosa

Xylella fastidiosa is a fastidious, xylem-limited bacterium that causes a r ange of economically important plant diseases. Here we report the complete genome sequence of X. fastidiosa clone 9a5c, which causes citrus variegated chlorosis-a serious disease of orange trees. The genome comprises a 52.7% GC-rich 2,679,305-base-pair (bp) circular chromosome and two plasmids of 51 ,158 bp and 1,285 bp. We can assign putative functions to 47% of the 2,904 predicted coding regions. Efficient metabolic functions are predicted, with sugars as the principal energy and carbon source, supporting existence in the nutrient-poor xylem sap. The mechanisms associated with pathogenicity a nd virulence involve toxins, antibiotics and ion sequestration systems, as well as bacterium-bacterium and bacterium-host interactions mediated by a r ange of proteins. Orthologues of some of these proteins have only been iden tified in animal and human pathogens; their presence in X. fastidiosa indic ates that the molecular basis for bacterial pathogenicity is both conserved and independent of host. At least 83 genes are bacteriophage-derived and i nclude virulence-associated genes from other bacteria, providing direct evi dence of phage-mediated horizontal gene transfer.