HOSEPIPE-A WWW-HOSTED DATA MANAGEMENT AND ANALYSIS SYSTEM FOR STS CONTENT MAPPING PROJECTS

We have developed a data management system, 'HOSEpipe' (High Output ST S Evaluation pipeline) to aid sample tracking and data analysis in STS content mapping projects. The system is based around a World Wide Web (WWW) server that provides a number of pages including forms for samp le processing and data entry accessible via a standard WWW browser app lication. The system is split into two main modules: firstly, a sequen ce evaluation and annotation module that takes de novo sequence for a potential STS, screens it against existing STSs and DNA sequence datab ases, followed by appropriate primer sequence design; secondly, a modu le that handles YAC Library STS screening and includes facilities for both sample tracking and experimental data analysis. We present the de sign and rationale of the HOSEpipe system and its development to suppo rt a whole chromosomal physical mapping project. This software and des ign approach is potentially applicable to physical mapping projects of varying sizes and resolution and to similar projects, such as sample sequencing and the construction of sequence-ready maps.