HIGH-RESOLUTION MAPPING BY YAC FRAGMENTATION OF A 2.5-MB XP22 REGION CONTAINING THE HUMAN RS, KFSD AND CLS DISEASE GENES

The disease loci for X-linked Retinoschisis (RS), Keratosis follicular is spinulosa decalvans (KFSD), and Coffin-Lowry syndrome (CLS) have be en localized to the same, small region in Xp22 on the human X Chromoso me (Chr). To generate a high-resolution map of the available contig in this area, we have used the YAC fragmentation vectors pBP108/ADE2 and pBP109/ADE2 and generated fragmented YACs from a 2.5-Mb YAC (y939H7) spanning the mentioned disease gene candidate regions. Forty-seven fra gmented YACs were generated and analyzed, ranging in size from 170 kb to over 2400 kb. The resulting YAC fragmentation panel was used to con struct a detailed restriction map of the region and has been used to b in clones and markers. As a deletion panel, it will present a Valuable resource for further mapping.