Transformation of floral organs with GFP in Medicago truncatula

A high frequency of embryogenesis and transformation from all parts of flow ers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtain ed. Using this flower system, we obtained transgenic plants expressing prom oter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this method allows us to save time and to use a smaller greenhous e surface for the culture of donor plants. Southern hybridization showed th at the internal gfp fragment had the expected size and the number of T-DNA copies integrated in the plant genome varied between one and three. These d ata suggest that the presence of the GFP protein has no toxic effects, sinc e no rearrangement of the gfp reporter gene was detected in the regenerated plants.