In vitro shoot regeneration from leaf explants of Roman Chamomile

Murashige and Skoog (1962) medium supplemented with 1.0 to 4.5 mu M of BA a nd 1.0 mu M of NAA induced adventitious bud formation and shoot development in leaf explants of Roman Chamomile. A higher number of adventitious buds was observed at the proximal end of the explants. Plantlets were replicated and multiplied on MS medium supplemented with 2.25 mu M of BA and 0.6 mu M of IAA. Plantlets were rooted on MS medium supplemented with 0.5 mu M of I BA and successfully weaned in vivo. The plants grew to maturity with high u niformity and no morphological signs of somaclonal variation.