The novel insertion sequences IS1417, IS1418, and IS1419 from Burkholderiaglumae and their strain distribution

Three insertion sequences, IS1417, IS1418, and IS1419, were isolated from B urkholderia glumae (formerly Pseudomonas glumae), a gram-negative rice path ogenic bacterium, on the basis of their abilities to activate the expressio n of the neo gene of the entrap vector pSHI1063. The 1335-bp IS1417 element with 17-bp imperfect terminal inverted repents was found to be flanked by 5-bp direct repeats of the vector sequence. IS1418 is 865 bp in length and carries 15-bp inverted repeats with a target duplication of 3 bp. The 1215- bp IS1419 sequence is bounded by the 36-bp terminal inverted repeats of the element and 7-bp direct repeats of the vector sequence. IS1417 and 1S1418 belong to the IS2 subgroup of the IS3 Family and the IS427 subgroup of the IS5 family, respectively, whereas IS1419 does not appear to be a member of any known IS family. Southern blot analysis of DNAs from B. glumae field is olates indicated that those IS elements are widely distributed, but the hos t range of the three IS elements appears to be limited to B. glumae and som e other related species such as B. plantarii. The polymorphisms exhibited i n B. glumae isolates suggest that those elements are useful for molecular e pidemiological studies of B. glumae infections. (C) 2000 Academic Press.