Sequencing and characterization of the cryptic plasmid QpRS from Coxiella burnetii

Authors
Lautenschlager, S Willems, H Jager, C Baljer, G
Citation
S. Lautenschlager et al., Sequencing and characterization of the cryptic plasmid QpRS from Coxiella burnetii, PLASMID, 44(1), 2000, pp. 85-88
Citations number
19
Categorie Soggetti
Molecular Biology & Genetics
Journal title
PLASMID
ISSN journal
0147619X → ACNP
Volume
44
Issue
1
Year of publication
2000
Pages
85 - 88
Database
ISI
SICI code
0147-619X(200007)44:1<85:SACOTC>2.0.ZU;2-S
Abstract
Plasmid QpRS from Coxiella burnetii, isolate Priscilla Q177, phase I, has b een completely sequenced. DNA for sequencing was amplified with "extra-long " (XL) PCR. The size of the QpRS plasmid sequence was determined to be 39,2 80 bp, with a G + C content of 39.3%. Putative proteins associated with rep lication and recombination were identified. The sequence of QpRS plasmid wa s analyzed for shared and unique regions among C. burnetii plasmids. (C) 20 00 Academic Press.