Polyamines and thiols in the cytoprotective effect of L-cysteine and L-methionine on carbon tetrachloride-induced hepatotoxicity

Citation
W. Chen et al., Polyamines and thiols in the cytoprotective effect of L-cysteine and L-methionine on carbon tetrachloride-induced hepatotoxicity, AMINO ACIDS, 18(4), 2000, pp. 319-327
Citations number
25
Categorie Soggetti
Biochemistry & Biophysics
Journal title
AMINO ACIDS
ISSN journal
09394451 → ACNP
Volume
18
Issue
4
Year of publication
2000
Pages
319 - 327
Database
ISI
SICI code
0939-4451(2000)18:4<319:PATITC>2.0.ZU;2-0
Abstract
The relationship between cellular glutathione (GSH), protein-SH levels, and lactate dehydrogenase (LDH), with respect to the effect of polyamines on t he cytoprotective ability of L-cysteine and L-methionine, the most importan t components in the sulfur amino acid metabolic pathway, in carbon tetrachl oride (CCl4)-induced toxicity in isolated rat hepatocytes was studied. CCl4 induced a LDH release and decreased cellular thiols and polyamines levels but treatment with L-cysteine and L-methionine reversed these decreases. Tr eating with methylglyoxal bis-(guanylhydrazone), MGBG, an irreversible inhi bitor of S-adenosylmethionine decarboxylase, which is a key enzyme in sperm idine and spermine biosynthesis, and therefore used to deplete cellular pol yamines, prevented the protective effect of L-cysteine and L-methionine, bu t the addition of exogenous polyamines inhibited the influence of MGBG. The se results suggest that the cytoprotective effect of L-cysteine and L-methi onine in CCl4-induced toxicity were via maintenance of cellular polyamines, GSH and protein-SH concentrations and prevention of LDH leakage.