An approach to solventless sample preparation procedure for pesticides analysis using solid phase microextraction/supercritical fluid extraction technique

Solid phase microextraction (SPME)/supercritical fluid extraction (SFE) tec hnique was investigated to realize the approach to the true solventless sam ple preparation procedures for pesticides analysis. Pesticides in spiked wa ter samples were concentrated by SPME technique and were desorbed from the SPME fiber coating with supercritical carbon dioxide as the desorption solv ent, prior to their analyses with high performance liquid chromatography (H PLC). Preliminary experiments were performed in order to optimize the desor ption conditions of pesticides from the SPME fiber coating using supercriti cal carbon dioxide as the desorption medium. Subsequently, extraction condi tions of pesticides from spiked water samples with SPME technique were opti mized using the optimized desorption conditions. Limit of detection of some pesticides used in this study was lower than the Japanese regulatory limit s for environmental water in a golf course. Linearity was found between the tested concentration range between 0.1 and 5.0 mg 1(-1) with thr R.S.D. be tween 7.4 and 12.0%. (C) 2000 Elsevier Science B.V. All rights reserved.