New DNA modification strategies involving oxime formation

Authors
Cebon, B Lambert, JN Leung, D Mackie, H McCluskey, KL Nguyen, X Tassone, C
Citation
B. Cebon et al., New DNA modification strategies involving oxime formation, AUST J CHEM, 53(4), 2000, pp. 333-339
Citations number
18
Categorie Soggetti
Chemistry
Journal title
AUSTRALIAN JOURNAL OF CHEMISTRY
ISSN journal
00049425 → ACNP
Volume
53
Issue
4
Year of publication
2000
Pages
333 - 339
Database
ISI
SICI code
0004-9425(2000)53:4<333:NDMSIO>2.0.ZU;2-A
Abstract
A new method for the formation of covalently linked DNA conjugates is descr ibed. The method involves installation of a 5'-hydroxylamine nucleophile on to synthetic DNA and condensation with a suitable electrophilic carbonyl co mpound to form an oxime linkage. Various protection strategies for the hydr oxylamine group and their merits are discussed and the formation of an olig onucleotide-peptide conjugate is described.