Replication of classical infectious bursal disease virus in the chicken embryo related cell line

Infectious bursal disease (IBD) is an acute, highly contagious viral diseas e. The diagnosis of IBD depends on time-consuming and costly procedures, li ke virus isolation on chick embryos and histopathological examination, A do uble antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), immun operoxidase and reverse transcription polymerase chain reaction (RT-PCR) we re applied in this study to detect classical IBD virus (IBDV) after three b lind passages of the Lukert strain on chicken embryo related (CER) cell mon olayer after different periods of infection: 6, 12, 24 and 48 h, Cytophatic effects were most evident 12 h post-infection (p.i.) but were observed at 6 h p.i. The maximum discrimination between IBDV-infected and uninfected ce ll suspensions obtained by the use of DAS-ELISA for virus detection corresp onded to 0.597+/-0.02 and 0.010+/-0.01 after 12h p.i., respectively. The RT -PCR was performed using the set of primers A3.1 and A3.2 to amplify the VP 2 region of the IBDV genome, This molecular technique demonstrated that fro m 6 h p.i., it was possible to detect the viral RNA. The results show that the CER cell line can be used for classical IBDV propagation, confirmed by the DAS-ELISA, immunoperoxidase and RT-PCR assay.