Fluorogenic substrates for assay of chymosin

Authors
Starovoitova, VV Filippova, IY Lysogorskaya, EN Oksenoit, ES Lavrenova, GI
Citation
Vv. Starovoitova et al., Fluorogenic substrates for assay of chymosin, BIOCHEM-MOS, 65(6), 2000, pp. 713-717
Citations number
13
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMISTRY-MOSCOW
ISSN journal
00062979 → ACNP
Volume
65
Issue
6
Year of publication
2000
Pages
713 - 717
Database
ISI
SICI code
0006-2979(200006)65:6<713:FSFAOC>2.0.ZU;2-W
Abstract
The use of fluorogenic substrates with intramolecular fluorescence quenchin g as substrates for chymosin was studied. It was shown that chymosin hydrol yzes the Phe-Phe peptide bond. The effect of pH on the hydrolysis of substr ates by chymosin was investigated. The catalytic characteristics of the hyd rolysis of the fluorogenic substrates were obtained at the pH optima. The i nfluence of dimethylformamide on chymosin activity was studied.