Effect of different saccharides on viability of isolated microspores and androgenic induction in Zea mays

Sucrose, glucose, fructose, and melibiose in different concentrations and c ombinations in the induction media influenced the viability of the isolated maize microspores and the formation of multinuclear structures. The induct ion of multinuclear structures on media containing combination of sucrose, fructose and glucose was lower than on media only with sucrose. In media co ntaining melibiose alone or in combination with sucrose, no induction of mu ltinuclear structures was found, however, microspore viability was improved .