Citation
Sh. Lim et al., Primer design and optimization for RAPD analysis of Nepenthes, BIOL PLANT, 43(1), 2000, pp. 153-155
Citations number
7
Categorie Soggetti
Plant Sciences
Journal title
BIOLOGIA PLANTARUM
ISSN journal
00063134
→ ACNP
Volume
43
Issue
1
Year of publication
2000
Pages
153 - 155
Database
ISI
SICI code
0006-3134(2000)43:1<153:PDAOFR>2.0.ZU;2-4
Abstract
Primers with higher G+C content produced better random amplified polymorphi
c DNA (RAPD) profiles in Nepenthes. The occurrence of clustered G's and C's
in the center of the primer seemed also to influence the banding patterns.
It was also observed that for certain polymerases, the use of different bu
ffers other than that recommended by the manufacturer provided a better amp
lification profile for Nepenthes.