Pulse radiolysis study of rapid electron transfer from electron adduct andreducing OH adduct of dAMP to riboflavin and flavin adenine dinucleotide (FAD)

Rapid electron transfer from electron adduct and reducing OH adduct of 2'-d eoxyadenosine-5'-monophosphate acid (dAMP) to riboflavin (RF) and flavin ad enine dinucleotide (FAD) was studied using the time-resolved pulse radiolys is techniques. Both spectroscopic and kinetic analyses showed that transien t absorption spectrum of electron adduct or OH adduct of dAMP formed at fir st, and then changed to that of radical anion of RF or FAD after several mi croseconds of pulse. The evidence indicated that electron transfer from ele ctron adduct and reducing OH adduct of dAMP to RF or FAD did occur. From bu ildup or bleaching kinetics of radical anions of RF and FAD, the rate const ants for electron transfer were determined, respectively.